Synchronized reinjection and coalescence of droplets in microfluidics.

نویسندگان

  • Manhee Lee
  • Jesse W Collins
  • Donald M Aubrecht
  • Ralph A Sperling
  • Laura Solomon
  • Jong-Wook Ha
  • Gi-Ra Yi
  • David A Weitz
  • Vinothan N Manoharan
چکیده

Coalescence of two kinds of pre-processed droplets is necessary to perform chemical and biological assays in droplet-based microfluidics. However, a robust technique to accomplish this does not exist. Here we present a microfluidic device to synchronize the reinjection of two different kinds of droplets and coalesce them, using hydrostatic pressure in conjunction with a conventional syringe pump. We use a device consisting of two opposing T-junctions for reinjecting two kinds of droplets and control the flows of the droplets by applying gravity-driven hydrostatic pressure. The hydrostatic-pressure operation facilitates balancing the droplet reinjection rates and allows us to synchronize the reinjection. Furthermore, we present a simple but robust module to coalesce two droplets that sequentially come into the module, regardless of their arrival times. These re-injection and coalescence techniques might be used in lab-on-chip applications requiring droplets with controlled numbers of solid materials, which can be made by coalescing two pre-processed droplets that are formed and sorted in devices.

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عنوان ژورنال:
  • Lab on a chip

دوره 14 3  شماره 

صفحات  -

تاریخ انتشار 2014